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KMID : 1101320060380030173
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Korean Journal of Clinical Laboratory Science
2006 Volume.38 No. 3 p.173 ~ p.178
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Detection of Extended-Spectrum β-Lactamase Producing Klebsiella pneumoniae by Multiplex Polymerase Chain Reaction
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Yang Byoung-Seon
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Abstract
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The production of extended-spectrum β-lactamases (ESBLS) is the main mechanism of bacterial resistance to third-generation cephalosporins and monobactams, whose prevalence varies depending on the different geographical areas. In the last years it has increased notably to the point of being considered a health problem of great importance. The characterization of the ESBLs producing Klebsiella penumoniae strains present in clinical isolates is time-consuming. I describe here the development of a new system, which consists of a multiplex PCR. I found 51 K. pneumoniae strains to be presumptive strains ESBLs producers by clinical and laboratory standards institute (CLSI) guidelines. The double disc synergy test showed 47 positive K. pneumoniae, which were K. pneumoniae isolates. All ESBLs producing K. pneumoniae strains were resistant to antibiotic amikacin, gentamicin and ciprofloxacin. By multiplex PCR analysis, blaTEM gene in 17 strains 44 blaSHV genes and blaCTX genes in 33 strains were identified. In this study, the multiplex polymerase chain reaction (PCR) assay was a good method to detect and differentiate ESBLs producing K. penumoniae strains in clinical isolates.
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KEYWORD
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Klebsiella penumoniae, Extended-spectrum β-lactamases, Multiplex polymerase chain reaction
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